Mouse Rps3 ELISA Kit

Principle of the assay: RPS3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RPS3 antibody and an RPS3-HRP conjugate. The assay sample and buffer are incubated together with RPS3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RPS3 concentration since RPS3 from samples and RPS3-HRP conjugate compete for the anti-RPS3 antibody binding site. Since the number of sites is limited, as more sites are occupied by RPS3 from the sample, fewer sites are left to bind RPS3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RPS3 concentration in each sample is interpolated from this standard curve. Species : Mouse Storage: Store the whole ELISA kit at 4℃ Samples: Serum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids. Gene: Rps3 Uniprot AC: P62908; P17073; P47933; Intended Use: Mouse Rps3 ELISA Kitallows for the in vitro quantitative determination of Rps3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.